RESEARCH DEVELOPMENT FOR ADVANCED METHODS SOLUTIONS: AGRICULTURE, HORTICULTURE, NURSERY, AMENITY

PLANTAE SOLUTIONS

Terrae

Investigating plant microbies and interactions

PLANT FEEDBACK

MODEL PLANTS PHENOTYPING

PLANTS POSITIVE FEEDBACK means:

Something happened! A deeper investigation is possible.

MOLECULAR BIOLOGY

Through molecular biology, we can achieve different levels of information.

DNA QUANTIFICATION

Microbial biomass quantification via dsDNA

qPCR

Quantification of bacteria (16S), fungi (ITS) and archaea (16S)

PCR to quantify functional genes of interest (e.g. AmoA…)

METAGENOMIC

Biodiversity analysis of microbial community based on High Throughput Sequencing (HTS) of 16S rDNA and ITS.

ENZYMATIC ACTIVITIES

1. A further step is to test the activity of the enzymes associated to the rhizosphere

2. Soil enzymes are involved in biogeochemical cycles of soil carbon (C), nitrogen (N), sulfur (S) and phosphorus (P), which can be used as early sensitive indicators of environmental changes/input.

3. Some of the enzymes are:
α-glucosidase
β-glucosidase
β-galactosidase
Acid phosphatase
Alkaline phosphatase
Phosphatase
Arylsulfatase
N-acetyl-glucosaminidase
Arylamidase
Urease
Dehydrogenase

MICROBIAL COMMUNITIES

1. Community metabolic fingerprinting, or CMF, is effective in distinguishing spatial and temporal changes in microbial communities induced by environmental changes/inputs.

2. In detail, different carbon sources can be evaluated in order to investigate the Carbon utilization patterns of soil microbes